Search results for "Pemphigus spyrothecae"

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Molecular characterization of the leucine cluster in Buchnera PSY, primary endosymbiont of the aphid Pemphigus spyrothecae

2002

ABSTRACT Buchnera strains from most aphid subfamilies studied to date have been found to carry the leucine gene cluster ( leuA , - B , - C , and - D ) on a plasmid, an organization unique among bacteria. Here, however, we demonstrate a classical chromosomal location of the cluster in Buchnera sp. strain PSY from the aphid Pemphigus spyrothecae (subfamily Pemphiginae). The genes that flank leuABCD in Buchnera sp. strain PSY appear to be adjacent in the genome of Buchnera sp. strain APS, a strain carrying a leucine plasmid. We propose that the presence of a leucine plasmid predates the diversification of symbiotic Buchnera and that the chromosomal location observed in Buchnera sp. strain PSY …

DNA BacterialSubfamilyMolecular Sequence DataPemphigus spyrothecaeApplied Microbiology and Biotechnologysymbiotic bacteriaPlasmidBacterial ProteinsBuchneraLeucineplasmidGene clusterevolutionInvertebrate MicrobiologyAnimalsgeneticsCloning MolecularSymbiosisGeneHydro-LyasesGeneticsBase SequenceEcologybiologyStrain (chemistry)Gene Amplificationbiochemical phenomena metabolism and nutritionbiology.organism_classificationPRI BioscienceMultigene FamilyLeucinebiosynthesisBuchneraPemphigusFood ScienceBiotechnologyanthranilate synthase trpegApplied and Environmental Microbiology
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Plasmid-encoded anthranilate synthase (TrpEG) in Buchnera aphidicola from aphids of the family pemphigidae

1999

Aphids are dependent on an intracellular symbiont (Buchnera aphidicola, Proteobacteria) for normal growth and reproduction (7, 19, 45). The bacteria reside in specialized cells in the aphid hemocele and are transmitted maternally through infection of eggs or embryos (11, 26). Phylogenetic studies have revealed two major characteristics of the evolutionary history of the association (37, 39); (i) the symbiosis had a single origin, dated about 150 million to 250 million years ago; and (ii) host and symbiont lineages have since diverged strictly in parallel. The association, like other symbioses in insects feeding on restricted and unbalanced diets, is thought to have a nutritional basis (5–7,…

Molecular Sequence DataPemphigus spyrothecaePolymerase Chain ReactionApplied Microbiology and BiotechnologyEvolution MolecularPlasmidPhylogeneticsInvertebrate MicrobiologyAnimalsAmino Acid SequenceRepliconCloning MolecularSymbiosisPhylogenyAnthranilate SynthaseDNA PrimersGeneticsAphidBacteriaBase SequenceSequence Homology Amino AcidEcologybiologyfood and beveragesAphididaebiochemical phenomena metabolism and nutritionPhysical Chromosome Mappingbiology.organism_classificationBiochemistryAphidsbiology.proteinAnthranilate synthaseBuchneraPlasmidsFood ScienceBiotechnology
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